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Update

PathoFinder launches second kit for COVID19 detection

In addition to the RealAccurate® Quadruplex  Corona-plus PCR Kit, PathoFinder launches the RealAccurate® Quadruplex  SARS-CoV-2 PCR Kit (CE-IVD), which detects viral RNA from nasopharyngeal swabs and  simultaneously targets the N gene and RdRp gene of the virus, allowing  sensitive detection and confirmation of positive results at once.

 

The kit is validated on the five most prevalent real-time instruments:  LightCycler480 II (Roche), Rotor-Gene Q  (Qiagen), MIC qPCR Cycler (BMS), CFX96 (Biorad) and Quantstudio 5 (Thermo  Fisher Scientific).

In combination with the RealAccuarate® Quadruplex SARS-CoV-2  PCR Kit, an unique extraction-free protocol has been developed. The robust composition of the RAQ SARS-CoV-2 PCR Kit allows replacement of a full nucleic acid extraction  procedure by a simple heat-treatment. This decreases sample processing time  and saves on nucleic acid extraction reagents and disposables.

Although,  Ct/Cp values obtained with the heat-treated samples are slightly higher than  those obtained with regular nucleic acid extraction, overall a nearly  comparable sensitivity for SARS-CoV-2 detection is obtained using  heat-treatment.

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