SmartFinder®: Multiplex PCR Technology Based on Melt Curve Analysis

SmartFinder

The SmartFinder® technology is PathoFinder’s proprietary state-of-the-art multiplex PCR technology for Real Time Molecular Diagnostics. It enables Single tube Multiplex Amplification in Real Time (SMART) of up to 22 infectious pathogens in a single clinical sample.

The SmartFinder® technology uses target specific probes, which, after a ligation step, are amplified and detected in Real Time. The pathogen identification is based on Melting Curve analysis in different detection channels (FAM, ROX and Cy5). This reaction is preceded by a target specific amplification to ensure high specificity and sensitivity.

Features and benefits:

  • Detection and differentiation of up to 22 pathogens in a single assay
  • As sensitive as monoplex Real Time PCR
  • Diagnosis within 4 hours after nucleic acid  extraction
  • Compatible for Lightcycler 480 (Roche), Lightcycler II (Roche), Rotor-Gene
     3000 or 6000 (Corbett) or Rotor-Gene Q (Qiagen) RealTime PCR instruments.
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MultiFinder®: Multiplex PCR Technology Based on DNA Fragment Size Analysis

MultiFinder

The MultiFinder® technology is PathoFinder's first generation of multiplex PCR technology. The MultiFinder® technology enables the detection and differentiation of up to 22 viral or bacterial targets in a single molecular diagnostic assay.

The MultiFinder® technology uses target specific probes, which, after a ligation step, are amplified and detected by size fractionation. The pathogen identification is based on size fractionation by capillary electrophoresis of the different ligated amplified probes. This reaction is preceded by a target specific amplification to ensure high specificity and sensitivity.

Features and benefits:

  • Detection and differentiation of up to 24 pathogens in a single assay
  • As sensitive as monoplex Real Time PCR
  • Diagnosis within 6 hours after nucleic acid  extraction
  • Compatible with CE systems of ABI and Beckman Coulter.
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RealAccurate®: mono- and duplex RealTime PCR kits

RealAccurate

PathoFinder also offers a number of Real Time PCR assays. These products contain optimized and validated sets of primers and probes for Real Time PCR. Using an uniform PCR protocol, RealAccurate® enables you to analyse multiple parameters at the same time in the same instrument. The RealAccurate® product line focuses the detection of  respiratory viruses and bacteria, including mycobacterium tuberculosis.

Features and benefits:

  • Standardized amplification conditions
  • Custom kits can be produced on request
  • Contains positive and internal controls
  • Diagnosis within 2 hours
Read more about RealAccurate