RespiFinder® 15 (RespiFinder)
- Adenovirus (AdV)
- Coronavirus 229E (Cor-229E)
- Coronavirus NL63 (Cor-NL63)
- Coronavirus OC43 (Cor-OC43)
- Human Metapneumovirus (hMPV)
- Influenza A (InfA)
- Influenza A/H5N1 (InfA/H5N1)
- Influenza B (InfB)
- Parainfluenza Type 1 (PIV-1)
- Parainfluenza Type 2 (PIV-2)
- Parainfluenza Type 3 (PIV-3)
- Parainfluenza Type 4 (PIV-4)
- Respiratory Syncytial Virus A (RSVA)
- Respiratory Syncytial Virus B (RSVB)
- Rhinovirus (RhV)
The RespiFinder® 15 kit (previous name: RespiFinder) is a multiplex PCR test which can detect and differentiate 14 RNA viruses and 1 DNA virus in a single assay.
Detection of the various amplicons is accomplished by size-fractionation on a capillary electrophoresis system like the DNA sequencers ABI310, ABI3100, ABI3130, ABI3730, ABI3500 (Applied Biosystems) and Beckman CEQ systems.
- 15 viral pathogens in one assay
- As sensitive as monoplex Real Time PCR
- Contains a competitive internal amplification control
- Diagnosis within 6 hours
- Validated on QCMD panels
- CE-IVD labelled
Acute respiratory tract infection is the most widespread type of acute infection in adults and children. The number of pathogens involved (e.g. Influenza virus, RSV, Parainfluenza virus, Rhinovirus, Adenovirus etc.) is numerous.
The RespiFinder® 15 kit is using a new multiparameter technology and detection is based upon size fractionation. Diagnosis is performed within 6 hours. RespiFinder® 15 is a ready to use set of primers, probes and enzymes. As 14 out of 15 viral targets consist of RNA rather than DNA, the reaction is preceded by a reverse transcription step in which the viral RNA is converted into cDNA using target specific primer pairs.
The RespiFinder® 15 assay is based on our proprietary MultiFinder technology, which allows a highly complex analysis of up to 25 targets in a single reaction.
The RespiFinder® 15 assay includes a pre-amplification step which combines a reverse transcription step with a PCR to amplify the target DNA. Subsequently, a MLPA reaction is performed comprising of a probe hybridization step, a probe ligation step and a probe amplification step. Only two primers are used in the final PCR. Every target results in a PCR fragment of a specific length.
- Nasopharyngeal aspirate/lavage
- swabs
- bronchoalveolar lavage (BAL)
- sputa
The Internal Amplification Control is a RNA transcript from the encephalomyocarditis (EMC) virus and is supplied as a control for the RNA/DNA isolation, the RespiFinder® 15 assay and to check for possible PCR inhibitors.
Detection of the various amplicons is accomplished by size-fractionation on a capillary electrophoresis system like the DNA sequencers ABI310, ABI3100, ABI3130, ABI3730, ABI3500 (Applied Biosystems) and Beckman CEQ systems.
Quality Control for Molecular Diagnostics (QCMD) is an international External Quality Assessment (EQA) provider for molecular diagnosis of infectious agents.
PathoFinder has successfully participated in many EQA programs for respiratory pathogens. Details of these tests are available on request.
We will keep participating in QCMD programs to ensure the quality, sensitivity and specificity our products.
- 15 viral pathogens in one assay
- As sensitive as monoplex Real Time PCR
- Contains a competitive internal amplification control
- Diagnosis within 6 hours
- Validated on QCMD panels
- CE-IVD labelled
