RealAccurate™ Respiratory RT PCR v2.0
- Adenovirus (AdV)
- Coronavirus 229E (Cor-229E)
- Coronavirus OC43 (Cor-OC43)
- Human Metapneumovirus (hMPV)
- Influenza A (InfA)
- Influenza B (InfB)
- Parainfluenza Type 1 (PIV-1)
- Parainfluenza Type 2 (PIV-2)
- Parainfluenza Type 3 (PIV-3)
- Parainfluenza Type 4 (PIV-4)
- Respiratory Syncytial Virus A (RSVA)
- Respiratory Syncytial Virus B (RSVB)
- Rhinovirus/Enterovirus (RhV/EV)
The RealAccurate™ Respiratory RT PCR v2.0 kit is a combination of 9 singleplex and duplex Real Time PCR tests which can detect 13 RNA viruses and 1 DNA virus.
Amplification and detection can be performed on Real Time PCR instruments able to detect fluorescence in the FAM- and Yakima Yellow channels.
- Standardized amplification conditions for 14 viruses
- Differentiate between Inf A and Inf B, RSV-A and RSV-B, Cor-229E and Cor-OC43 as well as PIV-2 and PIV-4
- Contains a competitive internal amplification control
- Diagnosis within 2 hours
- Validated on QCMD panels
- CE labelled
- Available as subsets (upon request only)
Acute respiratory tract infection (RTI) is the most widespread type of acute infection in adults and children and is a significant cause of disease in immunocompromised patients. Viruses can cause acute RTI, and the number of causative agents is large as well as diverse. Real Time PCR tests provide a fast and accurate means of molecular diagnostics. The RealAccurate™ Respiratory RT PCR kit v2.0 is a combination of 9 singleplex and duplex Real Time PCR tests which can detect 13 RNA viruses and 1 DNA virus. Because of the use of 2 fluorescent labels, this assay can differentiate between Inf A and Inf B, RSV-A and RSV-B, Cor-229E and Cor-OC43 as well as PIV-2 and PIV-4.
The RealAccurate™ Respiratory RT PCR v2.0 assay combines a reverse transcription step with a PCR to amplify the target DNA. The kit provides primers and probes to assay 20 samples for 14 respiratory viruses. Enzymes are not provided. The amplification conditions for these 9 separate tests are identical, which means all assays can be performed simultaneously. Positive and amplification controls are provided as well.
- Nasopharyngeal aspirate/lavage
- swabs
- bronchoalveolar lavage (BAL)
The Internal Amplification Control is a RNA transcript from the encephalomyocarditis (EMC) virus and is supplied as a control for the RNA/DNA isolation and to check for possible PCR inhibitors.
Amplification and detection can be performed on Real Time PCR instruments able to detect fluorescence in the FAM- and Yakima Yellow channels.
Quality Control for Molecular Diagnostics (QCMD) is an international External Quality Assessment (EQA) provider for molecular diagnosis of infectious agents. PathoFinder has successfully participated in many EQA programs for respiratory pathogens in 2007 - 2011. Details of these tests are available on request. We will keep participating in QCMD programs to ensure the quality, sensitivity and specificity our products.
- Standardized amplification conditions for 14 viruses
- Differentiate between Inf A and Inf B, RSV-A and RSV-B, Cor-229E and Cor-OC43 as well as PIV-2 and PIV-4
- Contains a competitive internal amplification control
- Diagnosis within 2 hours
- Validated on QCMD panels
- CE labelled
- Available as subsets (upon request only)
